Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;52(2): e8209, 2019. tab, graf
Article
in En
| LILACS
| ID: biblio-984033
Responsible library:
BR1.1
ABSTRACT
Vegetable oils have been used for a plethora of health benefits by their incorporation in foods, cosmetics, and pharmaceutical products, especially those intended for skin care. This study aimed to investigate the cutaneous benefits of a vegetable oil blend (VOB) formulation and its fatty acid composition. The anti-inflammatory activity was studied in macrophages of RAW 264.7 cells by investigating the release of nitric oxide (NO), superoxide anion generation (O2-), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6). ABTS cation radical scavenging capacity assay, ferric reducing antioxidant potential (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and NO free radical scavenging assays were used to evaluate the antioxidant activity. VOB was tested for its ability to stimulate fibroblast proliferation and migration using the scratch assay, and antibacterial activity by the microdilution test. The fatty acid profile of a freshly prepared VOB formulation was determined by gas chromatography before and after accelerated stability testing. Chemical composition of VOB revealed the presence of oleic acid (C181n-9; 63.3%), linoleic acid (C182n-6; 4.7%), and linolenic acid (C183n-6; 5.1%) as major mono- and polyunsaturated fatty acids. No changes in the organoleptic characteristics and fatty acid composition were observed after the accelerated stability test. VOB 100 µg/mL reduced the healing time by increasing the total number of cells in the wounded area by 43.0±5.1% compared to the negative control group. VOB also suppressed the pro-inflammatory TNF-α and IL-6 cytokines, and NO and O2- production in lipopolysaccharide-stimulated macrophage cells. In conclusion, the VOB formulation contributed to the improvement of current therapeutic strategies for cutaneous applications in skin care.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Wound Healing
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Plant Oils
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Fatty Acids
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Anti-Inflammatory Agents
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Anti-Bacterial Agents
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Antioxidants
Limits:
Animals
Language:
En
Journal:
Braz J Med Biol Res
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Braz. j. med. biol. res
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Braz. j. med. biol. res. (Online)
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Brazilian journal of medical and biological research
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Brazilian journal of medical and biological research (Impresso)
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Rev. bras. pesqui. méd. biol
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Revista brasileira de pesquisas médicas e biológicas
Journal subject:
BIOLOGIA
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MEDICINA
Year:
2019
Document type:
Article
Affiliation country:
Country of publication: